Why when I send the output signal of my pickup to a PC/Mac by way of a Scarlett 2i2 USB audio interface, the sound I receive from my monitors/headphones is only from the high frequencies?

Please make sure you are using the instrument input and not the microphone or line inputs. The instrument input has 1.5 MΩ impedance and should work just fine. To use the other inputs you will need a preamp. Otherwise, an input/output impedance mismatch may occur.

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